Antibiotic Resistance of Escherichia Coli Isolated from Chicken in Malaysia

A BSTR ACT. Colibacillosis is an important disease affecting the poultry industry in many countries, caused by the Avian Pathogenic E. coli (APEC): it manifests as various clinical signs. It contributes significantly to economic loss for poultry farmers as a result of high mortality and morbidity in poultry. To overcome this, antibiotics have been widely used to eliminate E. coli infection in poultry farms in recent years. Treatment with antibiotics has been considered as a vital regimen to control E. coli infection at the farm level for many years. However, high frequency of antibiotic resistance of E. coli isolates from chicken has become the centre of attention due to public health importance. The aim of the present study is to determine the multidrug resistant profiles of E. coli strains isolated from chicken. E. coli isolates obtained from clinical cases were re-identified and classified by conventional methods. Multidrug resistant profiles against 13 different antibiotics of 125 E. coli isolates were determined by using disk diffusion method according to Clinical Laboratory Standard Institute (CLSI). Antibiogram revealed that 81.6% of the E. coli isolates showed multidrug resistant profiles to different antibiotics. Most of the E.coli isolates were highly resistant to erythromycin (52.8%), followed with tetracycline (52.0%), spectinomycin (39.2%), tr imethoprim (38.4%) and f lumequin (37.6%). Out of 125 isolates tested, 19.2% were resistant to more than eight antibiotics, with one isolates found to be multidrug resistant to most of antibiotics except polymyxin B. These findings also demonstrated that most of the isolates were susceptible to antibiotics commonly used for E.coli infections treatment in poultry with lowest resistant score against polymyxin B (92.8%) and colistin (92.0%). Moderate resistant profiles were observed towards amoxycilin (25.6%), apramycin (16%), kanamycin (8.8%) and streptomycin (8.0%). High percentage of multidrug resistance was found among the E. coli isolated from chicken as an indicator to more serious problems in animal health. Therefore, continuous surveillance of RE# MJVR 0001-2016